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Assembly characteristics of plant keratin intermediate filaments in vitro
Authors:MIN Guangwei  YANG Cheng  TONG Xiangjun  ZHAI Zhonghe  
Institution:(1) College of Life Sciences, Peking University, 100871 Beijing, China;(2) Department of Biological Science and Technology, Tsinghua University, 100084 Beijing, China
Abstract:After selective extraction and purification, plant keratin intermediate filaments were reassembledin vitro. Scanning tunneling microscope (STM) and transmission electron microscope (TEM) micrographs showed that acidic keratins and basic keratins can assemble into dimers and further into 10 nm filamentsin vitro. In higher mcation images, it can be seen that fully assembled plant keratin intermediate filaments consist of several thinner filaments of 3 nm in diameter, which indicates the formation of protofilaments in the assembly processes. One of the explicit features of plant keratin intermediate filaments is a 24–25 nm periodic structural repeat alone the axis of both the 10 nm filaments and protofilarnents. The periodic repeat is one of the fundamental characteristic of all intermediate filaments, and demonstrates the half staggered arrangement of keratin molecules within the filaments. Project supported by the National Natural Science Foundation of China (Grant No. 39370352) and the Doctor Foundation of Minishy of Education of China.
Keywords:intermediate Nament  keratin            in vitro assembly  scanning tunneling microscope (STM)
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