Pores formed in lipid bilayers and in native membranes by nodularin,a cyanobacterial toxin |
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| Authors: | M Spassova I R Mellor A G Petrov K A Beattie G A Codd H Vais P N R Usherwood |
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| Institution: | (1) Biomolecular Layers Department, Institute of Solid State Physics, Bulgarian Academy of Sciences, 72 Tzarigradsko Chaussee, BG-1784, Sofia, Bulgaria;(2) Department of Life Science, Nottingham University, NG7 2RD University Park, Nottingham, UK;(3) Department of Biological Sciences, University of Dundee, DD1 4HN Dundee, UK |
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| Abstract: | Nodularin (NODLN), a cyclic pentapeptide hepatotoxin from the cyanobacterium Nodularia spumigena, induces pores in bilayers of diphytanoyl lecithin (DPhL) and in locust muscle membrane. NODLN increases the surface pressure of a DPhL monolayer; except when the surface pressure of the monolayer is high when the toxin causes a reduction of this parameter. NODLN pores exhibit many open conductance states; the higher state probabilities increasing when the transmembrane pressure is increased. The results from these studies are discussed in terms of two models for a NODLN pore, a torroidal model and a barrel-stave model. The edge energy of the NODLN pore of 1.4× 10–12 J/m is determined.Abbreviations NODLN
Nodularin
- MCYST-LR
Microcystin-LR
- ADDA
3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid
- DPhL
diphytanoyl lecithin
Correspondence to: A. G. Petrov |
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| Keywords: | Patch-clamp Lipid monolayers Lipid bilayers Locust muscle membranes Nodularia spumigena Cyanobacterial toxins Nodularin Membrane pores Edge energy |
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