Adenylyl cyclase deficient cr-1 (Crisp) mutant of Neurospora crassa: Cyclic AMP-dependent nutritional deficiencies |
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Authors: | Héctor F. Terenzi João A. Jorge José E. Roselino Renato H. Migliorini |
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Affiliation: | (1) Faculdade de Medicina de Ribeirão Preto, Departamento de Fisiologia e Bioquimica, Universidade de São Paulo, 14100 Ribeirão Preto, São Paulo, Brasil, South America |
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Abstract: | The inability to synthesize cyclic AMP drastically affects the nutritional metabolism of Neurospora crassa. The adenylyl cyclase-less mutant cr-1 (crisp) did not utilize several carbon sources, including glycerol, mannitol, arabinose, and casaminoacids. However, in glucose or acetate it grew as well as the wild type. The following evidence suggested that these nutritional deficiencies were a direct result of the cr-1 mutation: (i), in crosses to wild type they segregated together with the crisp morphological marker; (ii), cyclic AMP added to the cr-1 mutant growth medium overcame the nutritional deficiencies; (iii), the cyclic AMP effect was specific for the crisp mutant, for it was not observed with the wild type, nor with a spontaneous glycerol-utilizing cr-1 strain. |
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Keywords: | Neurospora crassa Adenylyl cyclase Crips mutant Nutritional deficiencies Growth Fungi Cyclic AMP |
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