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Phospholipid metabolism in roots and microsomes of sunflower seedlings: Inhibition of choline phosphotransferase activity by boron
Institution:1. Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan;2. International Mass Imaging Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan;3. Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo, Japan;4. Department of Optical Imaging, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan;5. Department of Systems Molecular Anatomy, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan;6. Department of Anatomy, The University of Hong Kong, 6/F, William MW Mong Block 21 Sassoon Road, Pokfulam, Hong Kong SAR, China;1. Department of Oral and Maxillofacial Surgery, University Hospital Tuebingen, 72076 Tuebingen, Germany;2. Zyagnum AG, 64319 Pfungstadt, Germany;3. Biovis'' Diagnostik MVZ, 65555 Limburg an der Lahn, Germany;4. Cancer Research Center, Sanford-Burnham Medical Research Institute, La Jolla, CA, USA;1. Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom;2. Department of Orthopaedics, Freeman Hospital, Newcastle upon Tyne NE7 7DN, United Kingdom;1. Institut für Pharmazeutische und Medizinische Chemie der Westfälischen Wilhelms-Universität Münster, Corrensstr. 48, D-48149 Münster, Germany;2. NRW Graduate School of Chemistry, Germany;3. Organisch-Chemisches Institut der Westfälischen Wilhelms-Universität Münster, Corrensstr. 40, D-48149 Münster, Germany;1. Hebei Advanced Thin Film Laboratory, Department of Physics, Hebei Normal University, Shijiazhuang City 050024, People’s Republic of China;2. School of Sciences, Hebei University of Science and Technology, Shijiazhuang City 050018, People’s Republic of China;3. Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures and Jiangsu Provincial Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, People’s Republic of China;4. State Key Laboratory of Magnetism, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China
Abstract:The action of boron on phospholipid composition and synthesis in roots and microsomes from sunflower seedlings has been studied. The fatty acid composition and relative amounts of individual molecular species of phospholipids in roots and microsomes were very similar. In both the content of phospholipids was decreased and the relative levels of their component fatty acids changed by treatment with 50 ppm of boron. This concentration of boron in the culture medium was found to inhibit the in vivo 1-14C] acetate incorporation into root lipids and that of Me-14C] choline into phosphatidylcholine of root microsomes. Cytidine-5-diphospho (CDP)-Me-14C] choline incorporation into phosphatidylcholine of isolated microsomes was also inhibited by 50 ppm of boron when present in the growth medium of seedlings. These results indicate that the decrease in phosphatidylcholine labelling from 14C] choline observed when root microsomes were treated with boron would be caused by a decrease in CDP-choline phosphotransferase activity.
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