Genetic control of hydroxycinnamoyl-coenzyme a: Anthocyanidin 3-glycoside-hydroxycinnamoyltransferase from petals of Matthiola incana |
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| Affiliation: | 1. NUTECH School of Applied Sciences and Humanities, National University of Technology, Islamabad, 44000, Pakistan;2. Department of Mathematics, COMSATS University Islamabad, Sahiwal Campus, 57000, Pakistan;3. Department of Mathematics, Quaid-I-Azam University 45320, Islamabad, 44000, Pakistan;4. Nonlinear Analysis and Applied Mathematics (NAAM) Research Group, Department of Mathematics, Faculty of Science, King Abdulaziz University, P. O. Box 80257, Jeddah, 21589, Saudi Arabia;1. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, PR China;2. Xiangya Hospital of Central South University, Changsha 410008, PR China;3. School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, PR China;1. Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt;2. Faculty of Medicine KAU (Rabigh), Saudi Arabia;1. Chemical Biology Program, Chulabhorn Graduate Institute, Laksi, Bangkok 10210, Thailand;2. Laboratory of Medicinal Chemistry, Chulabhorn Research Institute, Laksi, Bangkok 10210, Thailand;3. Center of Excellence on Environmental Health and Toxicology, Commission on Higher Education, Ministry of Education, Bangkok, Thailand;1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China;2. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China |
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| Abstract: | Anthocyanidin 3-glucosides and 3-sambubiosides acylated with 4-coumarate or caffeate were identified in flower extracts of lines of Matthiola incana with wild-type alleles of the gene u. An enzyme activity was demonstrated catalysing the acylation of 3-glucosides and 3-sambubiosides with 4-coumarate or caffeate using the respective CoA esters as acyl donors. Anthocyanins glycosylated in both the 3- and 5-positions were not acylated. The enzyme exhibited an pH optimum at 6.5 and was inhibited by divalent ions, EDTA, diethylpyrocarbonate and p-chloromercuribenzoate. Accumulation of acylated 3-glycosides during bud development is correlated with acyltransferase activity. In confirmation of the chemogenetic work, acyltransferase activity was found only in enzyme extracts from flowers of lines with the wild-type allele u+. |
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