Effect of tiron on monohydroxy and o-dihydroxyphenolase activity of mushroom tyrosinase |
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| Institution: | 1. Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India;2. Department of Pharmacognosy, Varadaraja Institute of Pharmaceutical Education and Research, Tumkur 572102, Karnataka, India;3. Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty, India;4. Cellular Stress and Immune Response Laboratory, Centre for Cancer Biology, University of South Australia, Adelaide, Australia;5. Pharmacy, Swansea University Medical School, Singleton Park, Swansea University, Wales SA2 8PP, United Kingdom |
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| Abstract: | Tiron has a multiple effect on mushroom tyrosinase. At relatively low concentrations (up to 3.3 mM), Tiron extended the lag period of tyrosine hydroxylation appreciably, while at concentrations between 3.3 and 8.3 mM the lag period was shortened and approached that of the control. At concentrations above 10 mM, Tiron shortened the lag period of tyrosine hydroxylation compared with that of the control.Tiron, at relatively high concentrations (above 266 mM), inhibited the initial rate of dl-DOPA oxidation by mushroom tyrosinase and lowered the final level of dopachrome formed. Preincubation of mushroom tyrosinase with Tiron resulted in the inactivation of the enzyme, with 50 % inactivation of 650 μg enzyme occurring in the presence of 400 mM Tiron. |
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