The biosynthesis of sesquiterpenoid phytoalexins in suspended callus cultures of Nicotiana tabacum |
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| Affiliation: | 1. Affiliated Stomatology Hospital of Guangzhou Medical University, School of Basic Medical Science, Guangzhou Medical University, Guangzhou 511436, Guangdong, China;2. The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan 511518, Guangdong, China;3. State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Medical University, Guangzhou 510120, Guangdong, China;1. Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan;2. Center for Spintronics Research Network (CSRN), Tohoku University, Sendai 980-8577, Japan;1. Key Laboratory of Tobacco Chemistry of Yunnan Province, China tobacco yunnan industrial Co., Ltd., Kunming 650231, PR China;2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China |
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| Abstract: | Suspended callus cultures of Nicotiana tabacum treated with commercial cellulase (ex Trichoderma viride) incorporated sodium [1,2-13C2]acetate to give a13C-enrichment of ca 1.6% of the carbon content of debneyol.13C NMR data indicated that the angular methyl group in debneyol arises by migration from the C-10 position of a eudesmane-type intermediate.13C enrichments of 0.8% and 1.3% respectively were observed in phytuberol and phytuberin isolated from the cultures. |
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