Monoclonal antibodies for the analysis and purification of isopentenyladenine cytokinins |
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| Affiliation: | 1. Center for Research and Technology Transfer (CRETECH), Vietnam Academy of Sciences and Technology (VAST), Hanoi 10072, Viet Nam;2. Graduate University of Sciences and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 10072, Vietnam;3. Centre for Agricultural Research, Martonvásár 2462, Hungary;4. College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China;1. School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi''an, PR China;2. Gansu Institute for Drug Control, Lanzhou, PR China;3. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, PR China |
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| Abstract: | A series of monoclonal antibodies (McAb) have been generated which show a high affinity for the cytokinin, isopentenyladenosine (2iPA). The McAb show different specificities to a range of cytokinins in a radioimmunoassay and are suitable for the analysis of isopentenyladenine (2iP) and related compounds in plant extracts. One McAb, MAC 160, has a high cross-reactivity with several cytokinins and is a good candidate for use in group separation or the analysis of a number of cytokinins simultaneously after separation. When coupled to Sepharose 4B affinity support, the McAb can also be used as immunoaffinity matrices to purify and resolve cytokinins from plant extracts. The McAb have been used to demonstrate the presence of concentrations of 2iP in tissues of the moss, Physcomitrella patens which exceed those in its culture medium. |
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