Genomic fingerprints of Salmonella species generated with repetitive element sequence-based PCR |
| |
Authors: | SI Koh-Luar GHB Choo SLR Tan and CWK Tan |
| |
Institution: | (1) The authors are with the Department of Chemical Process & Biotechnology, Singapore Polytechnic, 500 Dover Road, Singapore, 139651, Republic of Singapore |
| |
Abstract: | We describe the use of repetitive element sequence-based PCR (rep-PCR) on the two repetitive sequences, REP and ERIC elements, to distinguish members of closely related Salmonella species. Within the species, ERIC–PCR showed a higher discriminative potential than REP–PCR, but by using a combination of the two PCR methods it was possible to distinguish all the isolates examined. The rep-PCR fingerprints of Salmonella organisms were distinctly different from some Gram-positive bacteria, for example Staphylococcus, Bacillus megaterium, and even the closely related Escherichia coli and Serratia marcescens. Identical fingerprints were observed with whole-cell preparations. Rapid specimen preparation has enhanced the value of rep-PCR in timely analysis of epidemiological relationships. |
| |
Keywords: | DNA fingerprinting ERIC (enterobacterial repetitive intergenic consensus) sequence PCR REP (repetitive extragenic palindromic) sequence Salmonella spp |
本文献已被 SpringerLink 等数据库收录! |
|