Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen |
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| Authors: | L.W.J. Baillie M. Johnson R.J. Manchee |
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| Affiliation: | Defence Microbiology Division, The Chemical and Biological Defence Establishment, Porton Down, Salisbury, Wiltshire, UK |
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| Abstract: | An asporogenous strain of Bacillus subtilis , IS53, transformed with plasmid pPA102, produces the protective antigen (PA) component of the tripartite toxin of B. anthracis . Addition of yeast extract was required to support growth and PA production in all the media examined. Protective antigen expression was down-regulated during exponential growth and extracellular proteases caused marked degradation of the mature protein. |
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