The mutagenic effect of nitrosoguanidine onMycobacterium phlei PA |
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Authors: | M Koní?ková-Radochová I Málek |
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Institution: | (1) Department of Bacterial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4 |
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Abstract: | The effect of nitrosoguanidine on the induction of three types of mutagenic changes inMycobacterium phlei PA was studied. The mutagenic changes included: change of prototrophy to auxotrophy, conversion of sensitivity to isoniazide
to resistańce and sensitivity to streptomycin to resistance. The induction of auxotrophic mutants was successful especially
when using NTG at a concentration of 1000 μg/ml. A total of 100 auxotrophs was obtained out of which only 13 were sufficiently
stable to be used in further studies. Amino acid requirements especially the glycine−(serine−) type characterized more than half of all auxotrophic mutants obtained. A group of mutants requiring purines also included
a high number of mutants. A considerable spontaneous reversion frequency was found in both groups of auxotrophs. The kinetics
of the induction of INH-resistant mutants by nitrosoguanidine at a concentration of 1000 μg/ml was studied and a high induction
of these mutants, particularly at high lethal effect of the mutagen found. The mutability of the STMr marker was relatively low in the present model microorganism as compared with the two markers mentioned earlier. |
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