Improved accumulation of betulin and betulinic acid in cell suspension culture of Betula pendula roth by abiotic and biotic elicitors |
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Authors: | Razieh Jafari Hajati Najmeh Ahmadian Chashmi Kamal Ghasemi Bezdi |
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Institution: | 1. Faculty of forest sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran;2. Traditinal Medicine Clinical Trial Research Center, Shahed University, Tehran, Iran;3. Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran;4. Agricultural research, Education and Extension Oranization (AREEO), Cotton Research Institute of Iran, Gorgan, Iran |
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Abstract: | AbstractBetulin (B) and betulinic acid (BA) are two triterpenes with diverse pharmacological and physiological actions. Elicitation of Betula pendula Roth cell cultures by elicitors is an excellent strategy to increase B and BA levels. Six abiotic and biotic elicitors were studied to improve accumulation of B and BA in the cell culture of B. pendula. The B and BA production in treated cells was verified by HPLC. The results showed the maximum growth index (7) on day 3 in cells treated with 0.5?mg L?1 chlorocholine chloride (CCC). The increased accumulation of BA in the cells treated with 200?mg L?1 of chitosan was found to be 5.9?×?(6.5?mg g?1 DW) higher over control cells. Treating the cells with 2?mg L?1 of CCC, after 7?days, led to 149.3× enhancement of B content (19.4?mg g?1 DW) over the controls. Production of this triterpenoid at a much shorter time with a much higher growth rate can be economic and lead to producing large amounts of B and BA for anti-cancer and HIV drugs preparation. |
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Keywords: | Betula pendula betulin betulinic acid cell viability elicitor growth index |
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