MEDIUM OPTIMIZATION OF ANTIFUNGAL ACTIVITY PRODUCTION BY Bacillus amyloliquefaciens USING STATISTICAL EXPERIMENTAL DESIGN |
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Authors: | Héla Mezghanni Saoussen Ben Khedher Slim Tounsi |
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Affiliation: | Team of Biopesticides (LPIP), Centre of Biotechnology of Sfax, University of Sfax , Sfax , Tunisia |
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Abstract: | In order to overproduce biofungicides agents by Bacillus amyloliquefaciens BLB371, a suitable culture medium was optimized using response surface methodology. Plackett–Burman design and central composite design were employed for experimental design and analysis of the results. Peptone, sucrose, and yeast extract were found to significantly influence antifungal activity production and their optimal concentrations were, respectively, 20 g/L, 25 g/L, and 4.5 g/L. The corresponding biofungicide production was 250 AU/mL, corresponding to 56% improvement in antifungal components production over a previously used medium (160 AU/mL). Moreover, our results indicated that a deficiency of the minerals CuSO4, FeCl3 · 6H2O, Na2MoO4, KI, ZnSO4 · 7H2O, H3BO3, and C6H8O7 in the optimized culture medium was not crucial for biofungicides production by Bacillus amyloliquefaciens BLB371, which is interesting from a practical point of view, particularly for low-cost production and use of the biofungicide for the control of agricultural fungal pests. |
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Keywords: | antifungal activity production Bacillus amyloliquefaciens improvement medium optimization Plackett–Burman design response surface methodology |
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