THROMBIN IMMOBILIZATION TO METHACRYLIC ACID GRAFTED POLY(3-HYDROXYBUTYRATE) AND ITS IN VITRO APPLICATION |
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| Authors: | Alper Akkaya Nurdan Pazarlioglu |
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| Institution: | 1. Biochemistry Department, Faculty of Science, Ege University , Bornova-Izmir , Turkey alper.akkaya@ege.edu.tr;3. Biochemistry Department, Faculty of Science, Ege University , Bornova-Izmir , Turkey |
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| Abstract: | Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding. |
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| Keywords: | bleeding graft polymerization immobilization methacrylic acid poly(3-hydroxybutyrate) thrombin |
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