ENHANCED PRODUCTION OF 6-AMINOPENICILLANIC ACID IN AQUEOUS METHYL ISOBUTYL KETONE SYSTEM WITH IMMOBILIZED PENICILLIN G ACYLASE |
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| Authors: | Shu-Guang Fang Tan Qiang Rui-Jiang Liu Xi-Ming Xu |
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| Institution: | 1. School of Pharmacy, Jiangsu University , Zhenjiang , P. R. China;2. Shanghai Prolife Biotechnology Co. Ltd. , Shanghai , P. R. China;3. Faculty of Pharmacy, Guangxi Traditional Chinese Medical University , Nanning , P. R. China;4. United Research Center of Jiangsu University and Sopo Xingda Pharmaceutical Co. Ltd. , Zhenjiang , P. R. China;5. School of Pharmacy, Jiangsu University , Zhenjiang , P. R. China |
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| Abstract: | Enzymatic hydrolysis of penicillin G for production of 6-amino-penicillanic-acid (6-APA) was achieved by using penicillin G acylase as catalyst in an aqueous-methylisobutyl ketone (MIBK) system. The optimization was carried out and it was found that the best conversion was improved 10% more than the aqueous system, which was obtained at the conditions: initial pH 8.0, 5.0% (W/V) substrate (penicillin G), and temperature at 35°C, and the ratio of aqueous and organic phase was 3:1. The stability of the biocatalyst was studied at the operational conditions. After 5 cycles of semi-batch reactions, the residual activity of penicillin G acylase was 69.2% of the initial activity. There was no apparent loss of the yield of product. This process has a potential application in the industrial scale production of 6-APA because it simplifies the process effectively. |
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| Keywords: | 6-aminopenicillanic-acid hydrolyzation penicillin acylase two-phase system |
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