Simultaneous Detection of Creatine and Creatinine using a Sequential Injection Analysis/Biosensor System |
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| Authors: | Raluca‐Ioana Stefan‐van Staden Rahel Girmai Bokretsion Jacobus F van Staden Hassan Y Aboul‐Enein |
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| Institution: | 1. Faculty of Chemistry , University of Bucharest , Bucharest, Romania iustinavanstaden@yahoo.com;3. Faculty of Chemistry , University of Bucharest , Bucharest, Romania;4. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division , National Research Center (NRC) , Cairo, Egypt |
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| Abstract: | Abstract Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi‐enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on‐line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10). |
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| Keywords: | Creatine Creatinine Amperometric biosensors Creatininase Creatinase Sarcosine oxidase Sequential injection analysis |
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