LARGE SCALE BIOSYNTHESIS OF GANGLIOSIDE ANALOGUES BY RERF-LC-AI CELLS CULTURED IN HYPERFlask |
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| Authors: | Yumiko Shimura Junya Suzuki Miho Muraoka Maria Carmelita Zulueta Kasuya Koji Matsuoka |
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| Institution: | 1. Japan Chemical Innovation and Inspection Institute , Tokyo , Japan;2. Institute of Industrial Science , University of Tokyo , Tokyo , Japan;3. Division of Material Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan |
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| Abstract: | The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was higher than yields obtained from 100-mm dishes. |
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| Keywords: | biocombinatorial synthesis cell culture ganglioside glycoside oligosaccharide saccharide |
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