Preparative Scale,High Resolution Purification of Low Molecular Weight DNA Fragments

Abstract

A commercially available continuous electro elution system has been used to separate and purify low molecular weight DNA fragments from polyacrylamide gels. This method has several advantages over previously reported methods for the recovery of DNA fragments from polyacrylamide gels. This technique, which gives very high recovery rates (80–95%), can be carried out on a relatively large scale and in a way that is not labour intensive. Data are presented for the purification of DNA fragments with molecular weights in the range 1–4 ± 10 (200–700 base-pairs), although the method is also applicable to larger molecular weight DNA fragments, RNAs and proteins.