Recombinant chromosomes of advanced backcross plants between Allium cepa L. and A. fistulosum L. revealed by in situ hybridization |
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| Authors: | A Hou E B Peffley |
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| Institution: | (1) Department of Plant and Soil Science, and the Institute of Biotechnology, Texas Tech University, Lubbock, TX79409, USA e-mail: epeffley@ttu.edu Fax: +1806-742-1898, US |
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| Abstract: | Cytological analysis of (Allium cepa L.×Allium fistulosum L.)×A. cepa L. F1BC3 plants revealed most plants were diploid with 16 chromosomes. Karyotypes of these plants showed recombinant chromosomes.
Fluorescence and genomic in situ hybridization patterns of interspecific F1 hybrid and F1BC3 plants revealed A. fistulosum chromosomes or chromosomal segments. A highly repetitive 376-bp DNA sequence and genomic DNA of A. fistulosum revealed similar telomeric hybridization sites when hybridized onto A. fistulosum chromosomes. Cytogenetic evidence showed that A. fistulosum DNA has recombined into the A. cepa genome.
Received: 20 October 1999 / Accepted: 11 November 1999 |
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| Keywords: | Fluorescence in situ hybridization Genomic in situ hybridization Interspecific introgression Japanese bunching onion Onion |
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