Preliminary studies on the involvement of glutathione metabolism and redox status against zinc toxicity in radish seedlings by 28-Homobrassinolide |
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| Affiliation: | 1. Colledge of Chemistry, Jilin University, Changchun, 130012, China;2. Institute of Theoretical Chemistry, Jilin University, Changchun, 130023, China;3. From the CNRS, Laboratoire de Physiologie Cellulaire et Végétale, UMR 5168, F-38054 Grenoble, France,;4. the Université Grenoble Alpes, F-38054 Grenoble, France,;5. the CEA, DSV, BIG, F-38054 Grenoble, France,;6. the INRA, LPCV, UMR 1417, F-38054 Grenoble, France, and;1. Graduate School of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan;2. Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan;3. Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572 Ibaraki, Japan |
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| Abstract: | The effect of exogenous application of 28-Homobrassinolide (HBR) on radish (Raphanus sativus L.) seedlings under zinc (Zn2+) stress on glutathione (GSH) production, consumption and changes in redox status was investigated. Zinc toxicity resulted in oxidative burst as evidenced by increased accumulation of hydrogen peroxide (H2O2) and malondialdehyde (MDA) content. These stress indices were significantly decreased by HBR supplementation. Under Zn2+ stress, GSH pool was decreased, while the contribution of oxidized glutathione (GSSG) to total GSH increased (GSSH/GSH ratio), this translated into significant reduction of GSH redox homeostasis. In addition, an increase of phytochelatins (PCs) was observed. In radish seedlings under Zn2+ stress, the activities of gamma-glutamylcysteine synthetase (γ-ECS), glutathione synthetase (GS), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and cysteine (Cys) levels increased but the activity of glutathione reductase (GR) decreased. However, application of HBR increased the GSH pool and maintained their redox ratio by increasing the enzyme activities of GSH biosynthesis (γ-ECS and GS) and GSH metabolism (GR, GPX and GST). The results of present study are novel in being the first to demonstrate that exogenous application of HBR modulates the GSH synthesis, metabolism and redox homeostasis to confer resistance against Zn2+ induced oxidative stress. |
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| Keywords: | 28-Homobrassinolide Glutathione metabolism Cysteine Phytochelatins Redox status Zinc stress CDNB" },{" #name" :" keyword" ," $" :{" id" :" kw0040" }," $$" :[{" #name" :" text" ," _" :" 1-chloro-2,4-dinitrobenzene Cys" },{" #name" :" keyword" ," $" :{" id" :" kw0050" }," $$" :[{" #name" :" text" ," _" :" cysteine DTNB" },{" #name" :" keyword" ," $" :{" id" :" kw0060" }," $$" :[{" #name" :" text" ," _" :" 5,5′-dithio-bis(2-nitrobenzoic acid) DTT" },{" #name" :" keyword" ," $" :{" id" :" kw0070" }," $$" :[{" #name" :" text" ," _" :" dithiothreitol GR" },{" #name" :" keyword" ," $" :{" id" :" kw0080" }," $$" :[{" #name" :" text" ," _" :" glutathione reductase GPX" },{" #name" :" keyword" ," $" :{" id" :" kw0090" }," $$" :[{" #name" :" text" ," _" :" glutathione peroxidase GSSG" },{" #name" :" keyword" ," $" :{" id" :" kw0100" }," $$" :[{" #name" :" text" ," _" :" glutatathione disulfide GSH" },{" #name" :" keyword" ," $" :{" id" :" kw0110" }," $$" :[{" #name" :" text" ," _" :" glutathione GS" },{" #name" :" keyword" ," $" :{" id" :" kw0120" }," $$" :[{" #name" :" text" ," _" :" glutathione synthetase GST" },{" #name" :" keyword" ," $" :{" id" :" kw0130" }," $$" :[{" #name" :" text" ," _" :" glutathione-S-transferase HBR" },{" #name" :" keyword" ," $" :{" id" :" kw0140" }," $$" :[{" #name" :" text" ," _" :" 28-Homobrassinolide OPT" },{" #name" :" keyword" ," $" :{" id" :" kw0150" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" o" },{" #name" :" __text__" ," _" :" -phthaldialdehyde PC" },{" #name" :" keyword" ," $" :{" id" :" kw0160" }," $$" :[{" #name" :" text" ," _" :" phytochelatin PMSF" },{" #name" :" keyword" ," $" :{" id" :" kw0170" }," $$" :[{" #name" :" text" ," _" :" phenyl methyl sulfonyl fluoride PVP" },{" #name" :" keyword" ," $" :{" id" :" kw0180" }," $$" :[{" #name" :" text" ," _" :" polyvinylpyrolidone zinc γ-ECS" },{" #name" :" keyword" ," $" :{" id" :" kw0200" }," $$" :[{" #name" :" text" ," _" :" gamma-glutamylcysteine synthetase |
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