Microscale purification of proteins by line immunoelectrophoresis: Application of the technique in protein biogenesis studies

A small-scale version of line immunoelectrophoresis in combination with immunoprecipitate excision is describeb as a rapid convenient technique to purify proteins on a micro scale in biogenesis studies. In the purification of pig small intestinal microvillar enzymes the method was found to be capable of a quantitative purification and to result in a higher state of purity than an isolation procedure using protein A-Sepharose. Since the method furthermore allows a simultaneous purification of several different protein antigens from the sample, it may be of interest as an alternative method to other procedures in the purification of proteins on a micro scale.