Fluorographic detection of tritrium-labelled proteins in immunoelectropherograms with the water-soluble fluor,sodium salicylate |
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| Authors: | Renate Manteuffel Ernst Weber |
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| Institution: | Zentralinstitut für Genetik und Kulturpflanzenforschung Gatersleben, Akademie der Wissenschaften der D.D.R., Corrensstrasse 3, DDR-4325 Gatersleben, G.D.R. |
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| Abstract: | A method is described for detecting by fluorography tritium-labelled proteins in immunoelectropherograms performed on agarose gels. The technique is based on the impregnation of immunoplates with sodium salicylate immediately after electrophoresis without the formation of a mixed agarose-polyacrylamide gel prior to processing the immunoelectropherograms for fluorography. Sodium salicylate, an inexpensive and water-soluble compound, has been found to serve as a satisfactory fluor for enhanced detection of radioactivity in agarose gels. |
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| Keywords: | fluorography immunoelectrophoresis plant seed proteins PPO 2 5-diphenyloxazol POPOP 1 4-di(2-(5-phenyloxazoly))benzene DMSO dimethylsulphoxide |
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