A method for high-performance liquid chromatographic screening of UV-positive components in urine eluate from sephadex G-10 and modifications for determination of urinary salicylic,salicyluric and gentisic acids

Acidified urine is chromatographed on a small Sephadex G-10 column, and UV-absorbing urine constituents in an appropriate eluate are screened by reversed-phase high-performance liquid chromatography. Chromatograms of morning-urine specimens from healthy adults show 14–19 peaks. The elution positions of eighteen common aromatic urine compounds have been established. Modifications for the determination of urinary salicylic, salicyluric and gentisic acids are also presented.