Comparison of methods for the determination of β-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents |
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Authors: | Gjalt W Welling |
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Institution: | Laboratorium voor Medische Microbiologie, Rijksuniversiteit, Oostersingel 59, 9713 EZ Groningen The Netherlands |
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Abstract: | β-Aspartylglycine is an indicator of the absence of bacterial enzymatic activity in the intestinal tract. This study describes and compares four methods — (1) dansylation with thin-layer chromatography, (2) ion-exchange chromatography, (3) thin-layer electrophoresis, (4) high-voltage paper electrophoresis — to determine the concentration of β-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents. |
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