Increased Levels of Neuron-Specific Enolase in PC 12 Pheochromocytoma Cells as a Result of Nerve Growth Factor Treatment |
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| Authors: | S. A. Vinores,P. J. Marangos&dagger ,A. M. Parma,&dagger ,G. Guroff |
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| Affiliation: | Section on Intermediary Metabolism, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, Bethesda, Maryland 20205, U.S.A.;Clinical Psychobiology Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20205, U.S.A. |
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| Abstract: | Abstract: Treatment of PC 12 pheochromocytoma cells with nerve growth factor (NGF) resulted in increased levels of neuron-specific enolase (NSE). Neither insulin, growth hormone, cytochrome c , nor sodium butyrate increased NSE levels. Epidermal growth factor (EGF) did increase NSE levels, although not to the same extent as NGF. As little as 1 ng/ml NGF induced the maximal increase in NSE. As PC 12 cells increased in density, the NSE levels increased even in untreated cells. |
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| Keywords: | Nerve growth factor Neuron specific enolase Neural differentiation Pheochromocytoma Cell culture |
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