Evolutionary processes possibly limiting the kinds of amino acids in protein to twenty: a review.

Only 20 of more than 250 biosynthetic amino acids are common (coded) constituents of contemporary protein. In this paper, several stages of evolution, both prebiotic and biotic, are examined for means by which other (non-proteinous) amino acids may have been selected against. Simulated prebiotic experiments indicate that some non-proteinous amino acids were present prebiotically, that they could be incorporated during the formation of prebiotic protein, and that they would function in such protein. Biotic selection is thus indicated.Non-proteinous amino acids currently are available via biosynthetic pathways for potential incorporation into bioprotein. Codon-anticodon interaction, peptidyl transferases, and elongation and termination factors of protein synthesis do not show the specificity needed to preclude non-proteinous amino acids. Highly specific recognition among amino acids, tRNAs, and activating enzymes is concluded to be why the kinds of amino acids in contemporary protein are limited to twenty.Some of several theories concerning the origin, nature and evolution of the genetic code can readily accommodate non-proteinous amino acids. Some evidence suggests that such amino acids were eventually eliminated from protein because they were less suitable than related proteinous amino acids. However, deterministic or “direct interaction” theories currently lack sufficient experimental support to answer how non-proteinous amino acids were precluded; such theories, being testable, probably have the most potential for providing an answer.