| 大鼠肺动脉平滑肌培养细胞内Ca~(2+)反应的多样性 |
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| 引用本文： | 李智,赵生卉,张庆利,太田隆英,小田岛肃夫.大鼠肺动脉平滑肌培养细胞内Ca~(2+)反应的多样性[J].中国组织化学与细胞化学杂志,1997(1). |
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| 作者姓名： | 李智 赵生卉 张庆利 太田隆英 小田岛肃夫 |
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| 作者单位： | 中国医科大学药理教研室，日本金泽医科大学第一病理教室 |
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| 摘 要： | 用Ｃａ２＋荧光色素Ｆｌｏｕ－３／ＡＭ负荷原代培养的大鼠肺动脉平滑肌细胞，在共聚焦激光显微镜下观察细胞内Ｃａ２＋对各种缩血管物质反应的非均一性。实验结果提示：各种Ｃａ２＋通道的反应与细胞培养时间相关。８０％以上的Ｃａ２＋贮库具有ＣＩＣＲＣａ２＋通道，在肺血管管平滑肌细胞可能存在一种只具有ＣＩＣＲ通道的Ｃａ２＋贮库，ＣＩＣＲ的Ｃａ２＋释放作用强于ＩＣＲＣａ２＋通道
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| 关 键 词： | 肺动脉 平滑肌 细胞培养 共聚焦激光显微镜 |
THE MULTIPLICITY OF INTRACELLULAR CALCIUM-HANDLING MECHANISM OF CULTURED VASCULAR SMOOTH MUSCLE CELLS IN PAT PULMONARY ARTERY |
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| Abstract: | Primary cultured vascular smooth muscle cells (VSMCs) in rat pulmonary artery were loaded with Ca fluorescent dye fluo-3/AM. The heterogeneous responses of intracellular calcium to several vasoactive stimulants were observed under confocal laser microscope. The results indicated that responses of different calcium channels are associated with culture time. VSMCs of pulmonary artery have calcium stores only with Ca-induced Ca release (CICR) channels. |
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| Keywords: | Pulmonary artery Smooth muscle Cell culture Confocal laser microscope |
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