| 人基因组YAC分子克隆库的构建及DMD基因YAC克隆的筛选 |
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| 引用本文： | 柴建华,顾杨洪. 人基因组YAC分子克隆库的构建及DMD基因YAC克隆的筛选[J]. 遗传学报, 1993, 0(4) |
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| 作者姓名： | 柴建华 顾杨洪 |
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| 作者单位： | 复旦大学遗传学研究所，复旦大学遗传学研究所 上海 200433，上海 200433 |
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| 摘 要： | 以pYAC4为载体,以正常人白细胞和含4条X染色体的细胞株GM1414为DNA源构建成人基因组YAC(Yeast Artificial Chromosome,酵母人工染色体)分子克隆库,已得到原始克隆近2万个,插入DNA片段长度在400—1000kb,从其中选出一组YAC克隆,它们含有DMD基因全部DNA顺序。
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| 关 键 词： | 酵母人工染色体 人类基因组 X染色体 DMD基因 |
The Construction of Human Genome YAC Library and Screening by Hybridization of Membrane High Density Spoted in Array |
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| Chai Jianhua Gu Yanghong. The Construction of Human Genome YAC Library and Screening by Hybridization of Membrane High Density Spoted in Array[J]. Journal of Genetics and Genomics, 1993, 0(4) |
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| Authors: | Chai Jianhua Gu Yanghong |
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| Abstract: | The human genomic YAC (Yeast Artificial Chromosome) library was constructed with human white blood cell and the cell line GM1414 containing 4 X chromosome DNA using pYAC 4 as vector. Twenty thousand YAC clones were obtained with insert size of 400-1000kb. A set of YACs containing entire DMD geneDNA sequence were selected. |
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| Keywords: | YAC (Yeast Artificial Chromosome) Human genome X chromosome DMD gene |
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