Autophosphorylation of yeast hexokinase PII |
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| Authors: | R Fernández P Herrero E Fernández T Fernández Y S López-Boado F Moreno |
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| Institution: | Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain. |
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| Abstract: | Autophosphorylation of hexokinase PII was studied using an enzyme purified from Saccharomyces cerevisiae. Incubation of this enzyme preparation with gamma-32P]ATP and Mn2+ or Mg2+ gave a phosphoprotein of molecular mass 58,000 which corresponded to hexokinase PII. D-Xylose stimulated autophosphorylation of hexokinase PII. Dilution of hexokinase PII over a 10-fold concentration range did not change the specific activity of hexokinase PII autophosphorylation suggesting that it may occur by an intramolecular mechanism. |
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