Electrochemical biosensors for biocontaminant detection consisting of carbon nanotubes,platinum nanoparticles,dendrimers, and enzymes |
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| Authors: | Ampornphan Siriviriyanun Toyoko Imae Naoki Nagatani |
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| Affiliation: | 1. Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC;2. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC;3. Department of Biotechnology and Applied Chemistry, Faculty of Engineering, Okayama University of Science, Okayama 700-0005, Japan |
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| Abstract: | The presented approach provides the advanced development of effective, rapid, and versatile electrochemical sensors for a small amount of analytes on potential, cheap, and disposable printed chips. The electrocatalytic activity of this biosensor revealed the feasible detection of hydrogen peroxide at low potential (∼0.09 V) and the detection of a biocontaminant inhibitor (organophosphorus pesticide) in a wide range of concentrations. This efficiency comes from the chemical immobilization of catalysts (Pt nanoparticles) and electron transfer-enlarging materials (carbon nanotubes) on an electrode. Especially, dendrimers raise the stable conjugation of enzymes (acetylcholinesterase/choline oxidase/peroxidase) as well as nanoparticles and carbon nanotubes on an electrode. |
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| Keywords: | Poly(amido amine) dendrimer Platinum nanoparticle Multiwalled carbon nanotube Enzyme reaction Electrochemical biosensor Inhibition reaction |
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