Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. |
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| Authors: | L De Vuyst E J Vandamme |
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| Affiliation: | Laboratory of General and Industrial Microbiology, Faculty of Agricultural Sciences, University of Gent, Belgium. |
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| Abstract: | Nisin production by Lactococcus lactis subsp. lactis NIZO 22186 was studied in batch fermentation using a complex medium. Nisin production showed primary metabolite kinetics: nisin biosynthesis took place during the active growth phase and completely stopped when cells entered the stationary phase. A stringent correlation could be observed between the expression of the prenisin gene (nisA) and the synthesis of the post-translationally enzymically modified and processed mature nisin peptide. Moreover, it seemed likely that nisin had a growth control function. A physiological link is proposed between sucrose fermentation capacity and nisin production ability. Carbon source regulation appears to be a major control mechanism for nisin production. |
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