| 宇佐美曲霉木聚糖酶基因xynⅡ在不同毕赤酵母中的分泌表达 |
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| 引用本文： | 周晨妍,邬敏辰,李东峰,王瑾,王武.宇佐美曲霉木聚糖酶基因xynⅡ在不同毕赤酵母中的分泌表达[J].生物加工过程,2008,6(2):38-42. |
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| 作者姓名： | 周晨妍 邬敏辰 李东峰 王瑾 王武 |
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| 作者单位： | 1. 江南大学,工业生物技术教育部重点实验室,无锡,214122
2. 江南大学,医药学院,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 将宇佐美曲霉E001的内切-1,4-木聚糖酶基因克隆到毕赤酵母表达载体pPIC9K中,得到重组质粒pPXY-NII,将其经SalⅠ线性化后分别转化2株毕赤酵母GS115和KM71,xynⅡ基因通过同源重组被整合到毕赤酵母染色体上,并处于酵母α因子的下游,经筛选获得阳性重组菌PXGL98(Mut+)和PXKL29(Muts)。该木聚糖酶基因在2株毕赤酵母中均实现了分泌表达。同时对工程菌的发酵条件进行了优化,在甲醇诱导下,PXGL98与PXKL29培养物上清液中的酶活力分别可达1156.92 U/mL和1646.03 U/mL。
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| 关 键 词： | 宇佐美曲霉 内切-1 4-木聚糖酶基因 毕赤酵母 分泌表达 |
| 文章编号： | 1672-3678(2008)02-0038-05 |
| 修稿时间： | 2007年7月16日 |
Secreted expression of Aspergillus usamii xylanase gene in different Pichia pastoris |
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| ZHOU Chen-yan,WU Min-chen,LI Dong-feng,WANG Jin,WANG Wu.Secreted expression of Aspergillus usamii xylanase gene in different Pichia pastoris[J].Chinese Journal of Bioprocess Engineering,2008,6(2):38-42. |
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| Authors: | ZHOU Chen-yan WU Min-chen LI Dong-feng WANG Jin WANG Wu |
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| Institution: | 1 (1.The Key Laboratory of Industrial Biotechnology of the Ministry of Education; Jiangnan University; Wuxi 214122; China; 2.School of Medicine; China); |
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| Abstract: | The endo-1,4-xylanase gene from Aspergillus usamii E001 was cloned into the Pichia pastoris expression vector,pPIC9K,the recombinant plasmid was named pPXYNII.The recombinant plasmid pPXYNII was linearized with SalⅠand then transformed into Pichia pastoris GS115 and KM71,respectively.The xynⅡgene was in frame integrated into the Pichia genome through homologous recombination.The recombinant Pichia pastoris GS115(PXGL98) and KM71(PXKL29) secreted functional endo-1,4-xylanase,and the enzymatic activities reac... |
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| Keywords: | Aspergillus usamii endo-1 4-xylanase gene Pichia pastoris secrected expression |
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