Cytochemical method for staining fish basophils |
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| Authors: | M. Tavares-Dias,&dagger |
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| Affiliation: | Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Amazons (UFAM), Av. General Rodrigo Octávio Jordão Ramos, 3000, Coroado I, 69077-000 Manaus, Amazonas, Brazil |
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| Abstract: | The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. Metachromasia was more identifiable through better preservation of the granules. The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil granules. |
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| Keywords: | blood cytochemistry freshwater fishes toluidine blue |
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