脱脂蛋白模型多肽与脂质体相互作用的高效液相色谱研究

应用凝胶过滤高效液相色谱，测定了两个两亲性多肽Ａｍｐ１和Ａｍｐ２进入磷脂酰甘油／磷脂酰胆碱脂双层的表观分配常数，并利用三硝基苯磺酸分析研究了与脂质体结合的多肽的氨基暴露状况。由结果推测，处于膜结合状态的多肽的氨基端是暴露于水相的；Ａｍｐ１与脂双层相互作用强于Ａｍｐ２，一方面表现为Ａｍｐ１比Ａｍｐ２埋膜较深，另一方面表现为Ａｍｐ１与脂双层的结合能力比Ａｍｐ２强，而主要表现在于后者。此外也发现两个多肽在缓冲液中处于几乎不存在暴露的氨基的聚合状态。

Interaction of Apolipoprotein Model Peptides with Liposomes: A High Performance Liquid Chromatography Study

The apparent partition constants of two amphiphilic peptides, Amp1 and Amp2, for partitioning into phosphatidylglycerol/phosphatidylcholine bilayer were measured using size exclusion high performance liquid chromatography. The exposed amino groups of vesicle bound peptides were studied by TNBS assay. It was proposed that their N terminals were exposed to the aqueous phase, and that the main explanation for the stronger interaction of Amp1 with lipid bilayer compared with Amp2 was its stronger lipid binding ability, though Amp1 was also buried deeper in the lipid membrane. It was also found that the two peptides were polymerized in buffer, with their amino groups almost totally buried within the polymers.