A simple and modified manometric method for measuring oxygen uptake rate of plant cells in flask cultures |
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| Authors: | Zhi-You Wen Jian-Jiang Zhong |
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| Affiliation: | (1) East China University of Science and Technology, Research Institute of Biochemical Engineering, 130 Meilong Road, 200237 Shanghai, China |
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| Abstract: | Summary A simple and convenient technique was developed based on the principle of Warburg manometric method to measure O2 uptake rate (OUR) and CO2 evolution rate (CER) of suspended cells in a shake flask culture. It was successfully applied to suspension cultures of rice (Oryza sativa) and Panax notoginseng cells, and some important bioprocess parameters, such as OUR, CER, respiratory quotient (RQ), specific OUR (SOUR) and specific CER (SCER), were quantitatively obtained. The measuring system is easy to operate, able to treat many samples simultaneously and is economical. |
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