Organization of centromeres in the decondensed nuclei of mature human sperm |
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| Authors: | Andrei O Zalensky John W Breneman Irina A Zalenskaya B R Brinkley E Morton Bradbury |
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| Institution: | (1) Department of Biological Chemistry, School of Medicine, University of California, 95616 Davis, CA, USA;(2) Texas Medical Center, Baylor College of Medicine, 77030 Houston, TX, USA;(3) Life Sciences Division, Los Alamos National Laboratory, 87545 Los Alamos, NM, USA |
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| Abstract: | The localization of centromeres in mature human sperm was shown by immunofluorescent labeling and nonisotopic in situ hybridization. In the decondensed nucleus structural elements (dimers, tetramers, linear arrays and V shape structures) formed by individual centromeres of nonhomologous chromosomes were observed. They organize the compact chromocenter, which was shown for nuclei decondensed to a low extent. The chromocenter is buried inside the nucleus; in contrast, telomeric regions of chromosomes were tentatively localized on the periphery. Thus, a gross architecture, which can influence selective unpackaging of the paternal genome upon fertilization, exists in human sperm. |
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