Deletion of pigR gene in Monascus ruber leads to loss of pigment production |
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Authors: | Nana Xie Qingpei Liu Fusheng Chen |
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Affiliation: | 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China 2. State Key Laboratory of Agricultural Microbiology, Wuhan, 430070, Hubei, China
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Abstract: | Pigments produced by Monascus are traditional food colorants and are widely used as dietary supplements. Since genes involving in pigment biosynthesis have not been reported, we describe the identification of a putative pigment-regulatory gene (pigR) obtained by molecular analysis of an albino strain of Monascus ruber M7. In the pigR-deleted strain (ΔpigR), neither the pigments nor pigR expression were detected by HPLC or reverse-transcription PCR, respectively, whereas the introduction of the pigR, together with a constitutive trpC promoter into ΔpigR, caused it to produce 5.4 U of red pigments/g dry mycelia, about 12-fold higher than Monascus ruber M7 (0.46 U/g dry mycelia). Thus pigR up-regulates pigment production in Monascus ruber M7. |
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