Coexpression of somatostatin-like immunoreactivity and catecholaminergic properties in neural crest derivatives: comodulation of peptidergic and adrenergic differentiation in cultured neural crest |
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| Authors: | J E García-Arrarás M Fauquet M Chanconie J Smith |
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| Affiliation: | 1. USDA, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, 68933-0166, USA;2. Department of Animal Science, South Dakota State University, Brookings, SD, USA;3. Department of Animal and Rangeland Science, New Mexico State University, Las Cruces, NM, USA;4. Texas A&M AgriLife, Overton, TX, USA;5. College of Agriculture, Arkansas State University, Jonesboro, AR, USA;1. University of Applied Sciences and Arts Northwestern Switzerland (FHNW), School of Life Sciences, Gründenstrasse 40, CH-4132 Muttenz, Switzerland;2. Commonwealth Scientific and Industrial Research Organisation (CSIRO), Land and Water Flagship, PMB2, Glen Osmond, 5064 South Australia, Australia;3. IRCCS – Istituto di Ricerche Farmacologiche “Mario Negri”, Environmental Biomarkers Unit, Department of Environmental Health Sciences, Via La Masa 19, I-20156 Milan, Italy;4. Swiss Federal Institute of Technology (ETH Zürich), Institute of Biogeochemistry and Pollution Dynamics, Department of Environmental System Sciences, CH-8092 Zürich, Switzerland;1. Ovarian Molecular Physiology Laboratory, Department of Physiology, Institute of Biosciences, Sao Paulo State University, Botucatu, Brazil;2. Neogen Assisted Reproduction Laboratory, Ribeirao Preto, Brazil;3. Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy;4. Biogenesi, Reproductive Medicine Centre, Monza, Italy |
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| Abstract: | In the avian embryo, somatostatin-like immunoreactivity (SLI) and adrenergic characteristics appear virtually simultaneously in the developing sympathetic nervous system and adrenal medulla. We have used double-labeling techniques to show that both properties coexist in the same cells. In the quail, not only do all somatostatin-containing cells in the adrenosympathetic system exhibit tyrosine hydroxylase immunoreactivity and possess catecholamines (CA), but this coexistence of the peptidergic and adrenergic phenotypes is already present very early in ontogeny. However, not all adrenergic cells express SLI. The development of sympathoadrenal precursors can be followed in vitro. Adrenergic precursor cells, obtained from the migrating neural crest, differentiate in culture into neuron-like cells that contain SLI and CA. This coexpression can be regulated by the same factors. For instance, corticosterone and progesterone increase SLI content and CA production in the neural crest cell cultures. The ontogeny of the autonomic lineage is discussed in the light of these results. |
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