Antimicrobial efficiency and stability of two decontamination solutions |
| |
Authors: | " target="_blank">Ingrida Smeringaiova Otakar Nyc Peter Trosan Jaroslav Spatenka Jan Burkert Jan Bednar Katerina Jirsova |
| |
Institution: | 1.Laboratory of the Biology and Pathology of the Eye, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine,Charles University and General University Hospital,Prague,Czech Republic;2.Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine,Charles University and General University Hospital,Prague,Czech Republic;3.Department of Clinical Microbiology, Second Faculty of Medicine,Charles University,Prague,Czech Republic;4.Department of Transplantation and Tissue Bank,Motol University Hospital,Prague,Czech Republic |
| |
Abstract: | Two decontamination solutions, commercially produced BASE?128 and laboratory decontamination solution (LDS), with analogous content of antibiotic and antimycotic agents, were compared in their antimicrobial efficiency and stability (pH and osmolarity). Both solutions were compared immediately after thawing aliquots frozen for 1, 3 or 6 months. Agar well diffusion method was used to test their antimicrobial efficiency against five human pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Enterococcus faecalis. The difference in the inhibition of growth between the two decontamination solutions was mostly not statistically significant, with few exceptions. The most pronounced difference between the LDS and BASE?128 was observed in their decontamination efficacy against E. coli and E. faecalis, where the LDS showed to be more efficient than BASE?128. The osmolarity value of LDS decreased with cold-storage, the osmolarity values of the BASE?128 could not be measured as they were below the range of the osmometer. Slight changes were found in pH of the less stable LDS solution, whose pH increased from initial value 7.36?±?0.07 to 7.72?±?0.19 after 6 m-storage. We verified that BASE?128 and LDS are similarly efficient in elimination of possible placental bacterial contaminants and may be used for decontamination of various tissues. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|