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Transformation of 3-chlorodibenzofuran by Pseudomonas sp. HH69
Authors:Hauke Harms  Heinz Wilkes  Volker Sinnwell  Rolf-Michael Wittich  Karl Figge  Wittko Francke  Peter Fortnagel
Institution:Abteilung Mikrobiologie, Universit?t Hamburg, F.R.G.
Abstract:The dibenzofuran-degrading bacterial strain Pseudomonas sp. HH69 showed high oxidative activity towards 3-chlorodibenzofuran (3CDF). During the co-metabolic turnover of 3CDF large amounts of 4-chlorosalicylate and temporarily small amounts of salicylate were excreted. Simultaneously a yellow colour appeared due to the excretion of two polar products. Conversion of 3CDF by a mutant, derived from Pseudomonas sp. HH69 and defective in 2,3-dihydroxybiphenyl-1,2-dioxygenase led to the formation of equal quantities of 4'-chloro-2,2',3-trihydroxybiphenyl (4'CTHBP) and 4-chloro-2,2',3-trihydroxybiphenyl (4CTHBP). Crude extracts of the wild type transformed 4'CTHBP to 4-chlorosalicylate, whilst 4CTHBP was transformed to salicylate. Hence, we propose a non-selective initial attack on both aromatic rings of 3CDF and a degradative pathway for the resulting chlorotrihydroxybiphenyls.
Keywords:3-Chlorodibenzofuran  Non-selective initial attack  Chlorotrihydroxybiphenyls  4-Chlorosalicylate  Salicylate
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