Construction, overexpression, and purification of Arthrobacter globiformis amine oxidase-Strep-tag II fusion protein |
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Authors: | Juda G A Bollinger J A Dooley D M |
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Affiliation: | Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. |
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Abstract: | The copper-containing amine oxidase from Arthrobacter globiformis has been expressed and purified as a fusion protein with a C-terminal Strep-tag II peptide. This tag facilitates the rapid purification of the enzyme on a large scale using the StrepTactin POROS medium. For example, we have demonstrated that 50 mg of protein can be obtained in 2 days from 2 L of Escherichia coli. The purified fusion protein displays turnover and spectroscopic properties that are essentially identical to those of the wild-type enzyme. Given the location of the C-terminus in four amine oxidase crystal structures, this strategy should be quite general for the rapid purification of amine oxidases from multiple sources. |
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