Different effects of the probe summarization algorithms PLIER and RMA on high-level analysis of Affymetrix exon arrays |
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Authors: | Yi?Qu Fei?He Email author" target="_blank">Yuchen?ChenEmail author |
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Institution: | (1) National Engineering Center for Biochip at Shanghai, Libing Rd. 151, Shanghai, 201203, China |
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Abstract: | Background Alternative splicing is an important mechanism that increases protein diversity and functionality in higher eukaryotes. Affymetrix
exon arrays are a commercialized platform used to detect alternative splicing on a genome-wide scale. Two probe summarization
algorithms, PLIER (Probe Logarithmic Intensity Error) and RMA (Robust Multichip Average), are commonly used to compute gene-level
and exon-level expression values. However, a systematic comparison of these two algorithms on their effects on high-level
analysis of the arrays has not yet been reported. |
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Keywords: | |
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