A miniaturized sandwich immunoassay platform for the detection of protein-protein interactions |
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| Authors: | Qiongming Liu Qing Chen Jian Wang Ying Zhang Ying Zhou Cong Lin Wei He Fuchu He Danke Xu |
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| Institution: | (1) State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, 102206, China;(2) Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China;(3) Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China |
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| Abstract: | Background Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein
complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the
most widely used method for PPI detection. However, this traditional large scale resin-based coIP is too laborious and time
consuming. To overcome this problem, we developed a miniaturized sandwich immunoassay platform (MSIP) by combining antibody
array technology and coIP methods. |
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