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Anonymous nuclear markers for cetacean species
Authors:A. R. Amaral  M. C. Silva  L. M. Möller  L. B. Beheregaray  M. M. Coelho
Affiliation:1.Centro de Biologia Ambiental, Departamento de Biologia Animal,Faculdade de Ciências da Universidade de Lisboa,Lisbon,Portugal;2.Department of Biological Sciences,Macquarie University,Sydney,Australia;3.School of Biological Sciences,Flinders University,Adelaide,Australia
Abstract:Here we report the development and characterization of 17 anonymous nuclear markers for cetacean species. These markers were isolated from a genomic library built from a common dolphin (genus Delphinus), and tested across several families within Cetacea. An average of 1 SNP per 272 bp was found in 10 anonymous markers screened for polymorphism within the genus Delphinus (total of 6,537 bp sequenced). These markers represent a significant addition to the set of tools used in genetic studies of cetaceans where population and species boundaries have to be inferred in order to implement proper conservation strategies.
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