Suppressor studies on ilvI mutants of Saccharomyces cerevisiae |
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Authors: | E Gundelach |
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Institution: | Institut für Strahlenbiologie, Universität Münster, 44 Münster, W. Germany |
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Abstract: | In order to answer the question whether with the X-ray induction of an achromatic lesion (a gap) an increase of the chromatid length is involved (e.g. by a localized failure of spiralization), the length ratios of 26 straight and 22 flexed metaphase chromosomes of Vicia faba were measured. (The length ratio is defined as the quotient between the length of the chromatid with a gap and that of its sister-chromatid without a gap.) Our results agree with the hypothesis that the induction of a gap does not increase the length of the chromatid. Remarkably in each of the 22 flexed chromosomes the gap was located at the “outer” chromatid. The bearing of our results on the nature of X-ray-induced achromatic lesions is discussed. |
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Keywords: | MNNG YEP yeast extract (1%) with peptone (2%) and glucose (2%) |
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