对同一细胞内DNA、RNA和蛋白含量相关测定的显微荧光光度法

用荧光染料DAPI、PyroninY和FITC分别染同一细胞内DNA、RNA和蛋白.在紫外光、绿光和兰光顺序激发后,用MPVⅡ显微荧光光度计测量反映单个细胞内DNA、RNA和蛋白含量的荧光强度.根据荧光发射光谱分析,每种染料荧光之间的干扰是可以忽略的.测量结果与FCM获得的结果是一致的.显微荧光光度术对单个细胞多参数相关测量的优点是简单、便宜,并可用于对活细胞获得形态学和定量细胞化学的组合信息.

QUANTITATIVE,CORRELATED DETERMINATION OF DNA,RNA AND PROTEIN CONTENT OF INDIVIDUAL CELL USING A MICROSPECTROFLUOROMETER

A cytochemical method was developed to differentially stain celluar DNA, RNA and Proteins with fluoroouromes DAPI,Pyronin Y and FITC respectively.The fluorescence intensity reflecting the DNA,RNA and Protein content of individual cell were mesured in a MPV II Microspectrofluorometer after sequential excitation by uv, green and blue light.The interference between the fluorescence of each dye is negligent according the fluorescent emission spectra analysis. The measurement results is consistent with the results obtained by FCM.The advantage of the correlated measurements of multiparameters in individual cells by a Microfluorometer are simpler and cheaper. It can be used in living cell to get the combinative measurement information of morphology and quantitative cytochemistry.