Biochemistry and ultrastructure of iridescent virus type 29 |
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| Authors: | Paul N Black Carol D Blair Ann Butcher John L Capinera George M Happ |
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| Institution: | 1. Department of Zoology, University of Vermont, Burlington, Vermont 05405, USA;2. Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523 USA;3. Department of Zoology and Entomology, Colorado State University, Fort Collins, Colorado 80523 USA |
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| Abstract: | Physical and chemical parameters of iridescent virus type 29, isolated from the mealworm, Tenebrio molitor, have been analyzed. The icosahedral capsid is 130–135 nm in diameter and is surrounded by a fringe of coarse filaments. The virus has a buoyant density in CsCl of 1.31 g cm?3 and contains 20 to 25 structural proteins as analyzed by isoelectric focusing and SDS-polyacrylamide gel electrophoresis. The DNA has a buoyant density in CsCl of 1.6874 g cm?3 indicating a G + C content of approximately 28%. The lipid components of this virus differ from those of the host cell; the virus contains about 80% cardiolipin and 20% phosphatidyl choline. |
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