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Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2 |
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| Authors: | Petersen Bent O Sára Margit Mader Christoph Mayer Harald F Sleytr Uwe B Pabst Martin Puchberger Michael Krause Eberhard Hofinger Andreas Duus Jens Ø Kosma Paul |
| Institution: | aDepartment of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, 2500 DK Valby, Denmark bCenter of Nanobiotechnology, University of Natural Resources and Applied Life Sciences, Gregor-Mendelstr. 33, A-1180 Vienna, Austria cDepartment of Chemistry, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria dLeibniz-Institute of Molecular Pharmacology, Robert-Rössle-Str. 10, D-13125 Berlin, Germany |
| Abstract: | The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-mannose, and 2-acetamido-2-deoxy-D-mannuronic acid. The primary structure of the acid-degraded polysaccharide--liberated by HF-treatment from the cell wall--was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-alpha-D-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-beta-D-glucopyranosyl units: Formula: see text]. |
| Keywords: | Geobacillus Secondary cell wall polymer NMR spectroscopy Mass spectrometry Polysaccharide structure |
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