3种检测吡咯喹啉醌的方法比较

目的:研究和比较3种检测吡咯喹啉醌(PQQ)的方法,确定各种方法的特点和适用范围。方法:设计和改进了3种检测PQQ的方法,分别为活性电泳法、光谱法和NBT-Gly法,探讨其检测限和线性范围、精密度、样品检测和加样回收率。结果:活性电泳法专一性好,具有很高的灵敏度,可检测到12.6 ng/mL PQQ,可靠但准确性较差;NBT-Gly法操作简便,可用于大量样品的检测,但重复性不佳;光谱法精密度较好,但样品中存在吸光物质时对检测结果影响较大。结论:活性电泳法、光谱法和NBT-Gly法均可用于PQQ的检测,活性电泳法适于培养上清等复杂样品的粗略定量,NBT-Gly法适于大量样品的检测,光谱法适于纯度较高的PQQ的定量。

Comparing Three Kinds of Pyrroloquinoline Quinone Detection Methods

Objective： To research the feature and application of 3 methods for pyrroloquinoline quinone（PQQ） detection.Methods： We designed and improved three methods of PQQ detection,which were Native-PAGE assay,spectroscopy detection,and NBT-Gly method.These methords would be evaluated by the detectability and lineage range,precision,sample detection and recovery rate.Results： Comparing the three methords,we found that Native-PAGE assay had great sensitivity which could detect the concentration of PQQ above 12.6 ng/mL,but only for a rough detection.NBT-Gly method was simple and convenient which could be used to detect a large number of samples,but its repeatability is poor（RSD5%）.Spectroscopy detection＇s precision was perfect but could be impacted by absorbance substances.Conclusion： Native-PAGE assay,spectroscopy detection and NBT-Gly method all can be used for PQQ detection.Native-PAGE assay is applicable to complex samples,such as supernatants.NBT-Gly method is more suitable for detecting a large number of samples,while spectroscopy detection is more fitable for the purified PQQ.