| 人类胚胎干细胞系H9培养和分化中细胞异质性的发现 |
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| 引用本文： | 田靖,岳文,范秋声,陈娜,刘晓杰,王洋,赵志虎,裴雪涛,陈惠鹏.人类胚胎干细胞系H9培养和分化中细胞异质性的发现[J].生物技术通讯,2011,22(4):474-479. |
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| 作者姓名： | 田靖 岳文 范秋声 陈娜 刘晓杰 王洋 赵志虎 裴雪涛 陈惠鹏 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
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| 摘 要： | 目的:在人类胚胎干细胞系H9培养和分化过程中探讨该细胞系的异质性。方法:对人类胚胎干细胞系H9进行体外未分化培养和诱导分化,鉴定其多潜能性和分化状态;在诱导其向拟胚体细胞的分化过程中,检测多潜能相关基因及分化特异基因的表达情况。结果:发现多潜能相关基因(Oct4、SOX2和Nanog)和种系特异性基因(Cdx2、Bachurary、SOX1、Fgf5和AFP)并不限于分别在未分化细胞和分化细胞中表达。结论:提示H9细胞系在培养过程中的非基因异质性现象,为进一步认识胚胎干细胞的自我更新和多潜能性提供了有意义的参考。
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| 关 键 词： | 人类胚胎干细胞 非基因异质性 培养 分化 |
Finding the Heterogeneity of Human Embryonic Stem Cell Line H9 in the Cell Culture and Differentiation |
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| TIAN Jing,YUE Wen,FAN Qiu-Sheng,CHEN Na,LIU Xiao-Jie,WANG Yang,ZHAO Zhi-Hu,PEI Xue-Tao,CHEN Hui-Peng.Finding the Heterogeneity of Human Embryonic Stem Cell Line H9 in the Cell Culture and Differentiation[J].Letters in Biotechnology,2011,22(4):474-479. |
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| Authors: | TIAN Jing YUE Wen FAN Qiu-Sheng CHEN Na LIU Xiao-Jie WANG Yang ZHAO Zhi-Hu PEI Xue-Tao CHEN Hui-Peng |
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| Institution: | TIAN Jing,YUE Wen,FAN Qiu-Sheng,CHEN Na,LIU Xiao-Jie,WANG Yang,ZHAO Zhi-Hu,PEI Xue-Tao,CHEN Hui-Peng Beijing Institute of Biotechnology,Beijing 100071,China |
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| Abstract: | Objective: To discover the characteristic of heterogeneity of human embryonic stem cells in the process of culture and differentiation.Methods: Human embryonic stem(hES) cell line H9 were cultured and characterized to maintain the state of pluripotency and differentiation.As hES cells were differentiated to embryonic body,some plutipotent and lineage specific genes were detected.Results: Pluripotent related genes,such as Oct4,SOX2 and Nanog,and lineage specific genes including Cdx2,Bachurary,SOX1,Fgf5 and A... |
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| Keywords: | human embryonic stem cells non-genetic heterogeneity culture differentiation |
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